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Cell Signaling Technology Inc fth1
Fth1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+fth1/FTH1+Rabbit+mAb/pmc12933830-167-3-4
Average 96 stars, based on 379 article reviews

fth1 - by Bioz Stars, 2026-10

96/100 stars

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Membrane:

Article Title: Polystyrene nanoplastics-induced lung epithelial cells ferroptosis promotes pulmonary fibrosis via YY1/FTL axis
Article Snippet: Following electrophoresis, the proteins were transferred onto a polyvinylidene difluoride (PVDF, Millipore Corporation, Billerica, #MA 01821, USA) membrane and then blocked at room temperature with 5 % skim milk. .. The membrane was incubated with rabbit anti-GPX4 (1:1000; Abways, #CY6959), rabbit anti-FTH1 (1:1000; Abways, #CY5648), rabbit anti-FTL (1:1000; Proteintech, #10727-1-AP), anti-α-SMA (1:1000; Abways, #CY5295), anti-COL1 (1:1000; Proteintech, #14695-1-AP), anti-YY1 (1:1000; Cell Signaling, #46395), and rabbit anti-β-actin (1:1000; Sigma, #sc-69879) antibodies and incubated at 4 °C overnight. .. Then, the membranes were incubated with a secondary antibody (goat anti-rabbit IgG from Sigma, #A0545, USA, 1:20000) at room temperature for 1 h. Finally, the protein bands were visualized and captured using the Tanon-5200 imaging system (Tanon Company, Shanghai, China).

Incubation:

other:

Article Title: USP9X-triggered ferroptosis mediates follicular atresia via deubiquitinating Beclin1 in chicken
Article Snippet: The following primary antibodies were used: mouse anti-USP9X (cat. no. sab2107379, Sigma-Aldrich), Rabbit anti-Beclin1 (cat. no. ab62577, Abcam), Rabbit anti-p62 (cat. no. 232145, Cell Signaling Technology), mouse anti-GPX4 (cat. no. ab252833, Abcam), anti-ACSL4 (cat. no. sab2100035, Sigma-Aldrich), rabbit anti-FTH1 (cat. no. 43935, Cell Signaling Technology), rabbit anti-ubiquitin (cat. no. ab134953, Abcam), mouse anti-Myc-tag (cat. no. 05-724, Sigma), mouse anti-FLAG-tag (cat. no. F3165, Sigma-Aldrich), rabbit anti-HA-tag (cat. no. ab9110, Abcam), mouse anti-GAPDH (cat. no. AB2302, Sigma-Aldrich).

Produced:

Article Title: Pseudorabies virus induces ferroptosis by disrupting iron homeostasis through activation of TfR1 and ferritinophagy
Article Snippet: .. The following primary antibodies were used: mouse anti-PRV-gB protein (produced in our laboratory), mouse anti-PRV-VP16 protein (produced in our laboratory), mouse anti-β-actin (Sigma-Aldrich, USA; no. A5441), mouse anti-TfR1 (Cell Signaling Technology, USA; no. 46222), mouse anti-TfR1 (Sigma-Aldrich, USA; no. MABC1765), rabbit anti-FTH1 (Cell Signaling Technology, USA; no. 3998), rabbit anti-IRP1 (Cell Signaling Technology, USA; no. 20272), rabbit anti-IRP2 (Cell Signaling Technology, USA; no. 37135), rabbit anti-HIF-1α (Cell Signaling Technology, USA; no. 14179), rabbit anti-HIF-1β (Cell Signaling Technology, USA; no. 5537), rabbit anti-Rab11a (Proteintech, USA; no. 20229-1-AP), mouse anti-NCOA4 (Santa Cruz Biotechnology, USA; no. sc-373739), rabbit anti-LAMP1 (Cell Signaling Technology, USA; no. 9091), rabbit anti-TAX1BP1 (Proteintech, USA; no. 14424-1-AP), rabbit anti-GSDME (Abcam, USA; no. ab215191), rabbit anti-GSDMD (Novus Biologicals, USA; no. NBP2-33422), rabbit anti-MLKL (Cell Signaling Technology, USA; no. 37705), and rabbit anti-p-MLKL (Affinity Biosciences, China; no. AF7420). .. Horseradish peroxidase (HRP)-labeled rabbit (Cat No. A0208) or mouse (Cat No. A0216) secondary antibodies were purchased from Beyotime (China).

Article Title: Pseudorabies virus induces ferroptosis by disrupting iron homeostasis through activation of TfR1 and ferritinophagy.
Article Snippet: .. The following primary antibodies were used: mouse anti-PRV-gB protein (produced in our laboratory), mouse anti-PRV-VP16 protein (produced in our laboratory), mouse antiβ-actin (Sigma-Aldrich, USA; no. A5441), mouse anti-TfR1 (Cell Signaling Technology, USA; no. 46222), mouse anti-TfR1 (Sigma-Aldrich, USA; no. MABC1765), rabbit anti-FTH1 (Cell Signaling Technology, USA; no. 3998), rabbit anti-IRP1 (Cell Signaling Technology, USA; no. 20272), rabbit anti-IRP2 (Cell Signaling Technology, USA; no. 37135), rabbit antiHIF-1α (Cell Signaling Technology, USA; no. 14179), rabbit anti-HIF-1β (Cell Signaling Technology, USA; no. 5537), rabbit anti-Rab11a (Proteintech, USA; no. 20229-1-AP), mouse anti-NCOA4 (Santa Cruz Biotechnology, USA; no. sc-373739), rabbit anti-LAMP1 (Cell Signaling Technology, USA; no. 9091), rabbit anti-TAX1BP1 (Proteintech, USA; no. 14424-1-AP), rabbit anti-GSDME (Abcam, USA; no. ab215191), rabbit anti-GSDMD (Novus Biologicals, USA; no. NBP2-33422), rabbit anti-MLKL (Cell Signaling Technology, USA; no. 37705), and rabbit anti-p-MLKL (Affinity Biosciences, China; no. AF7420). .. Horseradish peroxidase (HRP)-labeled rabbit (Cat No. A0208) or mouse (Cat No. A0216) secondary Month XXXX Volume 0 Issue 0 10.1128/jvi.00974-2513 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/j vi o n 05 S ep te m be r 20 25 b y 24 02 :8 00 :6 34 3: 97 1f :1 5d 4: 25 80 :d 71 c: 6a ee . antibodies were purchased from Beyotime (China).

Image Search Results

E12.5 VPA exposed induces male-biased ferroptosis-associated alterations in the mPFC. A Representative protein bands of GPX4, SLC7A11/xCT, ACSL4 and FTH1 in mPFC. B-E Statistical graph of protein levels of GPX4, SLC7A11/xCT, ACSL4 and FTH1. n = 5 per group. F Content of ferrous iron in the P24 mPFC. n = 3 per group. G GSH concentration and the ratio of GSH and GSSH in the P24 mPFC measured. n = 3 per group. H The MDA level in the P24 mPFC. n = 3 per group. I The SOD concentration in the P24 mPFC. n = 3 per group. J Representative immunofluorescence images of SLC7A11 (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of SLC7A11/xCT, n = 3 per group. K Representative immunofluorescence images of ACSL4 (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. L Representative immunofluorescence images of ROS (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. M Electron microscope of mitochondrial morphology in the P24 mPFC. The second row represents a partial enlargement of the first row, respectively. Scale bar = 2 μm (raw) / 500 nm (zoom). Values was presented as mean ± SEM and analyzed by one-way ANOVA. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: Biology of Sex Differences

Article Title: Sex differences in ferroptosis-related vulnerability to autism-like deficits in the adolescent medial prefrontal cortex following embryonic valproic acid exposure

doi: 10.1186/s13293-026-00982-x

Figure Lengend Snippet: E12.5 VPA exposed induces male-biased ferroptosis-associated alterations in the mPFC. A Representative protein bands of GPX4, SLC7A11/xCT, ACSL4 and FTH1 in mPFC. B-E Statistical graph of protein levels of GPX4, SLC7A11/xCT, ACSL4 and FTH1. n = 5 per group. F Content of ferrous iron in the P24 mPFC. n = 3 per group. G GSH concentration and the ratio of GSH and GSSH in the P24 mPFC measured. n = 3 per group. H The MDA level in the P24 mPFC. n = 3 per group. I The SOD concentration in the P24 mPFC. n = 3 per group. J Representative immunofluorescence images of SLC7A11 (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of SLC7A11/xCT, n = 3 per group. K Representative immunofluorescence images of ACSL4 (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. L Representative immunofluorescence images of ROS (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. M Electron microscope of mitochondrial morphology in the P24 mPFC. The second row represents a partial enlargement of the first row, respectively. Scale bar = 2 μm (raw) / 500 nm (zoom). Values was presented as mean ± SEM and analyzed by one-way ANOVA. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The membranes were then incubated overnight at 4 °C with the following primary antibodies: GPX4 (1:2000; Cat# A11243; Abclonal), FTH1 (1:5000; Cat# A19544; Abclonal), ACSL4 (1:1000; Cat#38493T; Cell Signaling Technology), xCT/SLC7A11 (1:1000; Cat# 28001T; Cell Signaling Technology), and β-actin (1:50000; Cat# 66009-1-Ig; Proteintech).

Techniques: Concentration Assay, Immunofluorescence, Fluorescence, Microscopy

E12.5 VPA induces ferroptosis-related signatures in the hippocampus without sex differences. A Representative protein bands of GPX4, SLC7A11/xCT, ACSL4 and FTH1 in hippocampus. B-E Statistical graph of protein levels of GPX4, SLC7A11/ xCT, ACSL4 and FTH1. n = 5 per group. F Content of ferrous iron in the P24 hippocampus. n = 3 per group. G GSH concentration and the ratio of GSH and GSSH in the P24 hippocampus measured. n = 3 per group. H The MDA level in the P24 hippocampus. I The SOD concentration in the P24 hippocampus. J Representative immunofluorescence images of SLC7A11 (red) and DAPI (blue) in hippocampus. Scale bar = 100 μm. Mean fluorescence intensity of SLC7A11, n = 3 per group. K Representative immunofluorescence images of ACSL4 (red) and DAPI (blue) in hippocampus. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. L Representative immunofluorescence images of ROS (red) and DAPI (blue) in hippocampus. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. M Electron microscope of mitochondrial morphology in the P24 hippocampus. The second row represents a partial enlargement of the first row, respectively. Scale bar = 2 μm (raw) / 500 nm (zoom). Values was presented as mean ± SEM and analyzed by one-way ANOVA. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: Biology of Sex Differences

Article Title: Sex differences in ferroptosis-related vulnerability to autism-like deficits in the adolescent medial prefrontal cortex following embryonic valproic acid exposure

doi: 10.1186/s13293-026-00982-x

Figure Lengend Snippet: E12.5 VPA induces ferroptosis-related signatures in the hippocampus without sex differences. A Representative protein bands of GPX4, SLC7A11/xCT, ACSL4 and FTH1 in hippocampus. B-E Statistical graph of protein levels of GPX4, SLC7A11/ xCT, ACSL4 and FTH1. n = 5 per group. F Content of ferrous iron in the P24 hippocampus. n = 3 per group. G GSH concentration and the ratio of GSH and GSSH in the P24 hippocampus measured. n = 3 per group. H The MDA level in the P24 hippocampus. I The SOD concentration in the P24 hippocampus. J Representative immunofluorescence images of SLC7A11 (red) and DAPI (blue) in hippocampus. Scale bar = 100 μm. Mean fluorescence intensity of SLC7A11, n = 3 per group. K Representative immunofluorescence images of ACSL4 (red) and DAPI (blue) in hippocampus. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. L Representative immunofluorescence images of ROS (red) and DAPI (blue) in hippocampus. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. M Electron microscope of mitochondrial morphology in the P24 hippocampus. The second row represents a partial enlargement of the first row, respectively. Scale bar = 2 μm (raw) / 500 nm (zoom). Values was presented as mean ± SEM and analyzed by one-way ANOVA. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Techniques: Concentration Assay, Immunofluorescence, Fluorescence, Microscopy

E12.5 VPA induces ferroptosis-related alterations in the striatum without sex differences. A Representative protein bands of GPX4, SLC7A11/xCT, ACSL4 and FTH1 in striatum. B-E Statistical graph of protein levels of GPX4, SLC7A11/xCT, ACSL4 and FTH1. n = 5 per group. F Content of ferrous iron in the P24 striatum. n = 3 per group. G GSH concentration and the ratio of GSH and GSSH in the P24 striatum measured. n = 3 per group. H The MDA level in the P24 striatum. I The SOD concentration in the P24 striatum. J Representative protein bands of GPX4, SLC7A11/xCT and FTH1 in mPFC, hippocampus and striatum. K-M Statistical graph of protein levels of GPX4, SLC7A11/xCT and FTH1. n = 5 per group. N Representative immunofluorescence images of SLC7A11/xCT (red) and DAPI (blue) in striatum. Scale bar = 100 μm. Mean fluorescence intensity of SLC7A11/xCT, n = 3 per group. O Representative immunofluorescence images of ACSL4 (red) and DAPI (blue) in striatum. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. P Representative immunofluorescence images of ROS (red) and DAPI (blue) in striatum. Scale bar = 100 μm. Mean fluorescence intensity of ROS, n = 3 per group. Q Electron microscope of mitochondrial morphology in the P24 striatum. Scale bar = 2 μm (raw) / 500 nm (zoom). Values was presented as mean ± SEM and analyzed by one-way ANOVA. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: Biology of Sex Differences

Article Title: Sex differences in ferroptosis-related vulnerability to autism-like deficits in the adolescent medial prefrontal cortex following embryonic valproic acid exposure

doi: 10.1186/s13293-026-00982-x

Figure Lengend Snippet: E12.5 VPA induces ferroptosis-related alterations in the striatum without sex differences. A Representative protein bands of GPX4, SLC7A11/xCT, ACSL4 and FTH1 in striatum. B-E Statistical graph of protein levels of GPX4, SLC7A11/xCT, ACSL4 and FTH1. n = 5 per group. F Content of ferrous iron in the P24 striatum. n = 3 per group. G GSH concentration and the ratio of GSH and GSSH in the P24 striatum measured. n = 3 per group. H The MDA level in the P24 striatum. I The SOD concentration in the P24 striatum. J Representative protein bands of GPX4, SLC7A11/xCT and FTH1 in mPFC, hippocampus and striatum. K-M Statistical graph of protein levels of GPX4, SLC7A11/xCT and FTH1. n = 5 per group. N Representative immunofluorescence images of SLC7A11/xCT (red) and DAPI (blue) in striatum. Scale bar = 100 μm. Mean fluorescence intensity of SLC7A11/xCT, n = 3 per group. O Representative immunofluorescence images of ACSL4 (red) and DAPI (blue) in striatum. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. P Representative immunofluorescence images of ROS (red) and DAPI (blue) in striatum. Scale bar = 100 μm. Mean fluorescence intensity of ROS, n = 3 per group. Q Electron microscope of mitochondrial morphology in the P24 striatum. Scale bar = 2 μm (raw) / 500 nm (zoom). Values was presented as mean ± SEM and analyzed by one-way ANOVA. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Techniques: Concentration Assay, Immunofluorescence, Fluorescence, Microscopy

Comparable Ferroptosis-Associated alteration in the mPFC Following E12.5 and PND14 VPA Exposure. A Representative protein bands of GPX4, SLC7A11/xCT, ACSL4 and FTH1 in mPFC. B-E Statistical graph of protein levels of GPX4, SLC7A11/ xCT, ACSL4 and FTH1. n = 5 per group. F Content of ferrous iron in the P24 mPFC. n = 3 per group. G GSH concentration and the ratio of GSH and GSSH in the P24 mPFC measured. n = 3 per group. H The MDA level in the P24 mPFC. n = 3 per group. I The SOD concentration in the P24 mPFC. n = 3 per group. J Observation of the morphology of brain nerve cells in the mPFC by HE staining. Scale bar = 100 μm. The second row shows Nissl staining of neuronal morphology in the mPFC by. Scale bar = 100 (raw)/20 (zoom) µm. The black arrow represents cytoplasmic vacuolization, nuclear pyknosis and poorly defined nucleoli. Statistical graph of nissl body in the mPFC. n = 3 per group. K Representative immunofluorescence images of SLC7A11 (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of SLC7A11, n = 3 per group. L Representative immunofluorescence images of ACSL4 (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. M Representative immunofluorescence images of ROS (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of ROS, n = 3 per group. N Electron microscope of mitochondrial morphology in the P24 mPFC. The second row represents a partial enlargement of the first row, respectively. Values was presented as mean ± SEM and analyzed by one-way ANOVA. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: Biology of Sex Differences

Article Title: Sex differences in ferroptosis-related vulnerability to autism-like deficits in the adolescent medial prefrontal cortex following embryonic valproic acid exposure

doi: 10.1186/s13293-026-00982-x

Figure Lengend Snippet: Comparable Ferroptosis-Associated alteration in the mPFC Following E12.5 and PND14 VPA Exposure. A Representative protein bands of GPX4, SLC7A11/xCT, ACSL4 and FTH1 in mPFC. B-E Statistical graph of protein levels of GPX4, SLC7A11/ xCT, ACSL4 and FTH1. n = 5 per group. F Content of ferrous iron in the P24 mPFC. n = 3 per group. G GSH concentration and the ratio of GSH and GSSH in the P24 mPFC measured. n = 3 per group. H The MDA level in the P24 mPFC. n = 3 per group. I The SOD concentration in the P24 mPFC. n = 3 per group. J Observation of the morphology of brain nerve cells in the mPFC by HE staining. Scale bar = 100 μm. The second row shows Nissl staining of neuronal morphology in the mPFC by. Scale bar = 100 (raw)/20 (zoom) µm. The black arrow represents cytoplasmic vacuolization, nuclear pyknosis and poorly defined nucleoli. Statistical graph of nissl body in the mPFC. n = 3 per group. K Representative immunofluorescence images of SLC7A11 (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of SLC7A11, n = 3 per group. L Representative immunofluorescence images of ACSL4 (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of ACSL4, n = 3 per group. M Representative immunofluorescence images of ROS (red) and DAPI (blue) in mPFC. Scale bar = 100 μm. Mean fluorescence intensity of ROS, n = 3 per group. N Electron microscope of mitochondrial morphology in the P24 mPFC. The second row represents a partial enlargement of the first row, respectively. Values was presented as mean ± SEM and analyzed by one-way ANOVA. ns > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Techniques: Concentration Assay, H&E Stain, Staining, Immunofluorescence, Fluorescence, Microscopy

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